Analysis of fecal short chain fatty acid concentration in miniaturedachshunds with inflammatory colorectal polyps

[摘要] Short chain fatty acids (SCFAs) play an important role in the maintenance of colonichomeostasis, and their depletion has been reported in various gastrointestinal disorders.Inflammatory colorectal polyps (ICRPs) are a recently recognized disease specific tominiature dachshunds (MDs), and fecal dysbiosis with a reduction of SCFA-producingbacteria has been reported with this disease. Therefore, this study was performed based onthe hypothesis that a reduced SCFA concentration associates with the development of ICRPs.We recruited 11 ICRP-affected MDs and 25 control MDs. Their fecal SCFA concentrations andbacterial proportions were quantified using high performance liquid chromatography andquantitative real-time PCR, respectively. The feces of ICRP-affected MDs contained loweramounts of propionic acid and lower proportions of Bifidobacterium thanthe feces of control MDs. Furthermore, fecal proportions ofBifidobacterium, Firmicutes and Lactobacillusexhibited significant positive correlations with fecal concentrations of total SCFAsand/or propionic acid; fecal Escherichia coli proportions correlatednegatively with fecal concentrations of total SCFAs, as well as acetic, propionic andbutyric acid. This result indicates an association between fecal dysbiosis and fecal SCFAconcentrations; these phenomena may contribute to ICRP pathogenesis in MDs. Potentialtherapeutic targeting of the reduced propionic acid concentration using probiotics,prebiotics or SCFA enemas merits further study.

[学科分类] 兽医学

[关键词] fermentative end product;high performance liquid chromatography;inflammatory colorectal polyp;microflora;miniature dachshund