Distribution of methicillin-resistant coagulase-positive staphylococci(MRCoPS) in a surgical unit and cystotomy operation sites in a veterinary teachinghospital

[摘要] This study aimed to investigate the spread of methicillin-resistant coagulase-positivestaphylococci (MRCoPS) among veterinary staff, hand-touch sites and surgical tissue duringcystotomy operations on cats and dogs that were patients, and to analyze the geneticrelatedness and antimicrobial resistance profiles of the isolates. Human and environmentalsamples were obtained from the nasal passageways of 12 surgeons and veterinary assistantsand from 29 hand-touch sites of instruments in operative units and subjected to bacterialisolation and enumeration. Swab samples were collected in triplicate from 29 dogs andthree cats at the site of incision, from the incision area, from the peritoneum duringsurgery and from the peritoneum before suture. MRCoPS were identified bymecA gene detection and characterized by their antibiogram profile,SCCmec type and pulsed-field gel electrophoresis. Twenty-fourstaphylococci were isolated, derived from one veterinary assistant, 12 operating roomfloor areas and hand-touch sites, three dogs and one cat. Methicillin-resistant S.pseudintermedius (MRSP) was found on an electric clipper and rebreathingcircuits in the operating room. Three dogs were positive for MRSP during surgery, and onemethicillin-resistant S. aureus (MRSA) was detected in a cat. All MRCoPSwere resistant to doxycycline, erythromycin, clindamycin and enrofloxacin, but no patientsdeveloped surgical site infections. According to their genotypic patterns, the clonesobtained from the environment and human sources differed from the animal clones. Despiteintensive hygienic management, a variety of MRCoPS clones were present within the surgicalunit and during surgery.

[学科分类] 兽医学

[关键词] dog;methicillin-resistant coagulase-positive staphylococci;Staphylococcus pseudintermedius;surgical patient;veterinary hospital