Rapid diagnosis of equine influenza by highly sensitive silver amplificationimmunochromatography system

[摘要] Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV,H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previouslyreported that some rapid antigen detection (RAD) tests are fit for diagnosing EI althoughtheir sensitivity is not optimal. Here, we evaluated the performance of the newlydeveloped RAD test using silver amplification immunochromatography (Quick Chaser Auto FluA, B QCA) to diagnose EI. The detection limits of QCA for EIVs were five-fold lower thanthe conventional RAD tests. The duration of virus antigen detection in the infected horseswas longer than the conventional RAD tests. We conclude that QCA could be a valuablediagnostic method for EI.

[学科分类] 兽医学

[关键词] diagnosis;equine influenza;rapid antigen detection;silver amplification