Dose-dependent effect of glucose on GLP-1 secretion involves sweet taste receptor in isolated perfused rat ileum [Rapid Communication]

[摘要] Luminal glucose is an important stimulus for glucagon-like peptide 1 (GLP-1) secretion from intestinal endocrine cells.However, the effects of luminal glucose concentration on GLP-1 secretion remain unknown.In this study, we investigated the effect of luminal glucose concentrations (3.5, 5, 10, 15, and 20 mmol/L) on GLP-1 secretion from isolated perfused rat ileum.Results showed that the perfusate glucose concentration dose-dependently stimulated GLP-1 secretion from isolated perfused rat ileum, which was eliminated by the sweet taste receptor inhibitor gurmarin (30 μg/mL), but not inhibited by phloridzin (1 mmol/L), a Na+-coupled glucose transporters inhibitor.We conclude that luminal glucose induced GLP-1 secretion from perfused rat ileum in a concentration-dependent manner.This secretion was mediated by sweet taste receptor transducing signal for GLP-1 release on the gut of rat.

[学科分类] 内分泌与代谢学

[关键词] Sweet taste receptor;Glucagon-like peptide 1;Glucose concentration;Rat;Ileum