Activity guided isolation of chemical constituents from the biologically active methanol extract of Euphorbia schimperi C. Presl

[摘要] In this study we investigated the chemical constituents of bioactive methanol extract ofEuphorbia schimperiC. Presl. For this the methanol extract was fractionated into 20, 40, 60, 80% MeOH in CHCl 3 , and 100% MeOH fractions respectively by vacuum liquid chromatography. Excision wound surface of the animals were topically treated with these fractions at a dose of 100 mg/kg body weight for twenty days. Povidone-iodine ointment was used as a reference drug. Wound contraction measurement and period of epithelialization were used to assess the effect of fractions on wound repairing. The 100% MeOH fraction treated animals achieved significant (p < 0.001) value by showing 100% wound contraction and minimum period of epithelization (17.75±0.47) on the 20 thday as compared to standard drug treated animals on the same day. The active 100% MeOH fraction was subjected to various chromatographic techniques led to the isolation of miquelianin ( 1 ), kaempferol 3- O -glucuronide ( 2 ) and quercitrin ( 3 ). Compounds ( 1-3 ) were isolated from this plant for the first time.                KEY WORDS :Euphorpia schimperiC. Presl, Wound healing, Chromatographic techniques, Chemical constituents Bull. Chem. Soc. Ethiop.2017 , 31(3), 471-479.  DOI:http://dx.doi.org/10.4314/bcse.v31i3.11.

[学科分类] 化学（综合）

[关键词] Euphorpia schimperi C. Presl;Wound healing;Chromatographic techniques;Chemical constituents