Resistance of western Cuba Aedes aegypti mosquitoes to three insecticide formulations

[摘要] Objective:determine the susceptibility of Aedes aegypti (L.) mosquitoes to threeinsecticide formulations using the impregnated bottle bioassay, standardizedby the Centers for Disease Control and Prevention (CDC).Methods:an evaluation was conducted of A. aegypti field populations collectedin Havana and Pinar del Río in relation to three of the insecticides mostcommonly used for A. aegypti control in Cuba: Galgotrin 25 EC (cypermethrin),Aqua k-othrine 2 EW (deltamethrin) and Clorcide 44 EC (chlorpyrifos) using theCDC impregnated bottle method. Analysis was also performed of three insecticideresistant reference strains and the Rockefeller susceptible reference strain.Determination was made of the time when the bottles may be effectively usedafter impregnation with each insecticide.Results:most of the field populations evaluated were found to be resistant to Aqua k-othrine2 EW and Clorcide 44 EC, and all of them showed susceptibility to Galgotrin25 EC. The reference A. aegypti strains resistant to temephos, deltamethrinand propoxur showed high resistance to the study formulations, whereas the Rockefellerstrain showed susceptibility. The bottles may be used effectively 4-15 daysafter impregnation, and be reused up to 5 times, depending on the storage time. Conclusions: the CDC bottle bioassay may be a useful tool to evaluate the susceptibilityof insecticide formulations in Cuban A. aegypti field populations. Galgotrin25 EC is still effective for A. aegypti control in Cuba.

[学科分类] 传染病学

[关键词] Aedes aegypti;CDC bottle bioassay;Cuba;insecticide resistance;surveillance;insecticide formulations.