Evaluation of the sensitivity of Faust method and spontaneous sedimentation for the diagnosis of giardiasis

[摘要] Introduction:in a routine clinical laboratory, parasitological methods are used for the detectionand diagnosis of intestinal parasitic diseases. Objective: To evaluate the sensitivity of Faust´s and the spontaneoussedimentation methods (Hoffman, Pons and Janer). Methods: a comparative study was developed. The analyzes were made oncontrol samples containing Giardia lamblia from the Laboratory of Clinical Pathologyof Santa Casa de Misericórdia of São Paulo and processed againin the clinical laboratory at the University of Nove de Julho by 18 analysts. Results: the statistical difference estimated by the McNemmar test (p=0.0156) and the Bland and Altman statistics through the graphical analysis showedlow correlation between the techniques applied in this research, which indicatesthat the Faust technique is the most sensitive for the detection of protozoancysts. Conclusions: this paper is relevant for the quality control of laboratoryprocedures to be applied to minimize the risk of false positives and false negatives.

[学科分类] 传染病学