IFN-xcexb1/IFN-xcexbb responses to respiratory viruses in paediatric asthma

[摘要] We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (e.g. IFN-xcexb1 and IFN-xcexbb) and their signal transduction; at baseline and during disease exacerbation; in cohorts of pre-school children with and without asthma.At the time of recruitment into the Europe-wide study PreDicta; and during symptoms; NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged in vitro with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.We found that PBMCs from asthmatic children infected in vitro with the RV1b serotype upregulated MYD88; IRF1; STAT1 and STAT2 mRNA; whereas MYD88; IRF1; STAT1 and IRF9 were predominantly induced in control children. Moreover; during symptomatic visits because of disease exacerbation associated with RV detection in NPF; IFN-xcexb1 production was found increased; while IFN-xcexbb secretion was already induced by RV in asthmatic children at baseline.During asthma exacerbations associated with RV; asthmatic children can induce IFN-xcexb1 secretion; indicating a hyperactive immune response to repeated respiratory virus infection.

[学科分类] 呼吸医学