Proteomic comparison of biomaterial implants for regeneration of peripheral nerve tissue

[摘要] (cont.) There was no significant difference in TGF [beta]2 levels between treatment groups. Whole wound expression of a SMA was 440% greater in the silicone treatment group than in the collagen treatment group by immunoblot measurement. Immunofluorescent measurement indicated that a SMA expression in the ROI was 160% greater in silicone than in collagen treated wounds, with significant differences in the nerve stumps (proximal, p=.0243; distal, p=.0021). Proteomic comparisons suggest that collagen tubes are more effective at promoting nerve regenerate than silicone tubes due to heightened levels of TGF 03, less [alpha] SMA expression, and possibly decreased levels of TGF p1 at early stages of wound healing. Trends in protein differences observed in nerve wounds treated with regenerative versus nonregenerative devices are consistent with differences observed in wounds in the early fetal versus adult healing stages. Results from this study support early fetal regeneration as a model for induced regeneration in the peripheral nerve.

[发布机构] Massachusetts Institute of Technology