Evaluation of the Ki-67 and MCM3 Expression in Cytologic Smear of Oral Squamous Cell Carcinoma

[摘要] Statement of the Problem:Squamous cell carcinoma (SCC) is the most frequent oral cancer whose 5-year survival rate is 80% for early-detected lesions and nearly 30-50% for advanced lesions. Early detection of oral cancers and precancerous lesions can improve the patient’s survival and decrease the morbidity. Purpose:This study aimed to evaluate and compare the Ki-67 and MCM3 expression in cytologic smear of oral SCC (OSCC). Materials and Method:We examined 48 oral brush biopsies including 28 OSCC and 20 healthy non-smoking samples. Immunocytochemistry staining was performed for Ki-67 andMCM3 by using an EnVision-labeled peroxidase system, and labeling index (LI) was calculated. Results:Out of 28 OSCC cases, 27(96.4%) cases contained MCM3 positive cells and 22(78%) cases contained Ki-67 positive cells. All normal mucosa were Ki-67 and MCM3 negative. MCM3 and Ki-67 LI were significantly higher in OSCC than normal mucosa ( p < 0.001). MCM3 LI was significantly higher than Ki-67 LI in OSCC group ( p < 0.001). Conclusion:Immunocytologic evaluation of Ki-67 and MCM3 can be used for early detection of OSCC. Furthermore, MCM3 may be a more sensitive cytologic biomarker than Ki-67 in SCC patients.  Keywords ●Squamous Cell Carcinoma ●Cytology ●Biopsy ●Ki-67 antigen ●Minichromosome Maintenance Complex Component 3

[学科分类] 口腔科学

[关键词] Squamous Cell Carcinoma ;Cytology ;Biopsy ;Ki-67 antigen ;Minichromosome Maintenance Complex Component 3