Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

[摘要] Venoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros. The crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide fraction (

[学科分类] 药理学

[关键词] Peptide;Hypotension;Viperinae