RP-HPLC AND HPTLC METHODS FOR THE ESTIMATION OFRILUZOLE IN TABLET DOSAGE FORM

[摘要] Two simple, specific, accurate and precise methods, namely, Reverse Phase High Performance Liquid Chromatography and high performance thin layer chromatography were developed for estimation of Riluzole in tablet dosage form. For the HPLC method, Promosil C-18, 5 µm column consisting of 200 × 4.6 mm i.d in isocratic mode, with mobile phase containing 25 mM KH2PO4 buffer (pH 2.8) : acetonitrile: (40:60 v/v) was used. The flow rate was 1 ml/min and effluent was monitored at 265 nm. The retention time was found to be 6.7 min. For the high performance thin layer chromatographic method a Camag system comprising of  Linnomat V automatic sample applicator, Hamilton syringe, Camag TLC Scanner-3, Camag WinCATs software with stationary phase precoated silica gel 60F254 and mobile phase consisting of chloroform : ethyl acetate : methanol (4:5.9:0.1 v/v/v) were used. The detection of spot was carried out at 265 nm. The Rfvalue was found to be 0.75. The methods were validated in terms of linearity, accuracy and precision. The linearity curves were found to be linear over 35-115 µg/ml for high performance thin layer chromatography and 100-900 ng/spot for high performance thin layer chromatography. The proposed methods were successfully used for estimation of Riluzole in tablet dosage form.

[学科分类] 基础医学

[关键词] Riluzole;HPLC;HPTLC;validation;analysis