CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF SERRATIA SP. FROM COMPOST BY THE USE OF 16SRIBSOMAL RNA GENE SEQUENCE

[摘要] Serratia bacterium was isolated from the compost samples and it was identified as Serratia sp., based on microscopic and biochemical characteristics. For molecular identification using specific PCR, the chromosomal DNA was extracted from the purified isolate and the DNA was subjected to amplification of the 16S r RNA gene. The PCR product was about 1200bp, the obtained amplicone was sequenced. The sequence analysis revealed that the isolate belongs to S. marcescens isolates with similarity 95%. The DNA sequence was deposited in gene bank under accession number of KP 797836 and the strain was labeled as SerratiamarcescensFek 323. The phylogentic analysis showed that our isolate is more closely related to chinease S.marcescens strain.

[学科分类] 药理学

[关键词] Aml S Owis;Ghazal FM;Abd EL-Wehab;AF;Mekhemar GAA;Microbial biosurfactant- Emulsification – Identification of Bacilli isolates.