Bisphenol-A induced damage in testicular structure and its amelioration by Vitamin E and Tinospora cordifolia

[摘要] Present study was aimed to investigate the effect of Bisphenol-A (BPA) on testicular tissue of goat and its reversal by antioxidant Vitamin E and Tinospora cordifolia. Testicular tissue culture was carried for 4 and 8 hours. In Group 1, testicular tissue was exposed to two doses of BPA (10¬-2 and 102 nM/ml) while in Group 2 and 3, different doses of BPA (10¬-2,102 nM/ml)were supplied along with Vitamin E (0.1µM/ml) and Tinospora cordifolia (250µg/ml), respectively. It was observed that BPA induced significant damage in the testicular cells. Various degree of histomorphological alterations observed were: reduction in population of spermatids, spermatozoa, vacuolization in lumen, damage in seminiferous epithelium, pyknosis in germ cells and reduced number of luminal spermatozoa. Supplementation with Vitamin E and Tinospora cordifolia¬ resulted in reduction in vacuolization, desquamation of germ cells in lumen and decline in pyknotic cells. Population of sperm cells were elevated and significant restoration in shape of seminiferous tubules was observed as compared to treatment groups. It is concluded that Vitamin E (antioxidant) and Tinospora cordifolia restores fertility in BPA exposed goat testis and exhibit protective effect against BPA induced damage in testis in vitro.

[学科分类] 药理学

[关键词] Rajnesh K Sharma;Ambika G;hi;BPA;Tinopsora cordifolia;Vitamin E;spermatids;spermatozoa.