Radical Scavenging and Teratogenic Activities of Stem-Bark, Leaves, and Fruit Rind of Guyabano (Annona muricata Linn.)

[摘要] This work reported the radical scavenging activity and the toxic and teratogenic effects of stem-bark, leaves, and fruit rind extracts of Annona muricata Linn. in Danio rerio embryos. Stem-bark extract recorded the highest radical scavenging activity of 87.92%, followed by fruit-rind (78.64%) and leaves (75.87%) extracts. In D. rerio assay, 100% mortality was noted in embryos at 1000 μg/ml and higher concentrations of stem-bark and 5000 μg/ml and higher concentrations of leaves and fruit rind extracts after 48 hours of exposure. Delayed development and malformations were determined as teratogenic parameters. Embryos at 500 μg/ml of stem-bark and 1000 μg/ml of both leaves and fruit rind and their higher concentrations recorded 100% delayed development. Similarly, these concentrations of the three extracts showed 100% malformations. However, higher concentrations did not show abnormalities due to arrested growth at earlier periods of observations. Extract treated zebrafish showed hook-like tail, bent tail, malformation of the eyes, less pigmentation, scoliosis, and yolk deformity. The toxic and teratogenic effects of A. muricata were dependent on the parts of the plant, concentration of the extracts and time of exposure

[学科分类] 药理学

[关键词] Rich Milton R. Dulay;Jocelyn S. De Vera;Annona Muricata;Antioxidant;Teratogen;Zebrafish;Free Radicals;Embryo-Toxic.