ESTABLISHMENT AND CHARACTERIZATION OF A FI BROBLAST CELL LINE DERIVED FROM GANSU ALPINE FI NE-WOOL SHEEP -

[摘要] ABSTRACT: Afibroblast cell line was successfully established from ear marginal tissue of Gansu alpine finewool sheep by using a primary explant technique and cell cryogenic preservation technology. Cryopreserved cells were checked for quality and biological characteristics and showed typical fibroblast morphology when cultured again in vitro. The growth curve was typically S-shaped as the cell population passed through a lag phase, a logarithmic phase and a plateau phase. Biological analysis sho.

[学科分类] 仪器