Electrically Evoked Synaptosomal Amino Acid Transmitter Release in Human Brain in Alcohol Misuse

[摘要] Severe chronic alcohol misuse leads to neuropathological changes in human brain, with the greatest neuronal loss in the dorsolateral prefrontal cortex. In this region, GABA_A receptors are selectively upregulated, and show altered subunit expression profiles only in alcoholics without comorbid disease, whereas glutamate_NMDA subunit expression profiles are selectively downregulated only in alcoholics with comorbid cirrhosis of the liver. To determine whether these outcomes might be conditional on synaptic transmitter levels, evoked release was studied in well-characterized synaptosome suspensions preloaded with L-[³H]glutamate and [¹⁴C]GABA and stimulated electrically (±10 V contiguous square waves, 0.4 ms, 100 Hz, 1.5 min) with and without Ca²⁺. Stimulation elicited brief peaks of both radioisotopes that were larger in the presence of Ca²⁺ ions (p < 0.01). A repeat stimulus evoked a second, smaller (p < 0.01) peak. Ca²⁺-dependent L-[³H]glutamate release, but not [¹⁴C]GABA release, was higher overall in alcoholics than in controls (p < 0.05). With comorbid cirrhosis, L-[³H]glutamate release showed a graded response, whereas [¹⁴C]GABA release was lowest in noncirrhotic alcoholics. Release patterns did not differ between cortical regions, or between males and females. Neither age nor postmortem interval was a significant confounder. The released transmitters may differentially alter receptor profiles on postsynaptic cells.

[学科分类] 神经科学

[关键词] Brain damage;Excitotoxicity;Cirrhosis