Single wavelength check isocratic reverse phase HPLC method for fast and direct estimation of alkyl aromatic oxidation products

[摘要] An isocratic, reverse phase liquid chromatographic method has been developed for the quantitative determination of p-xylene oxidation products. The method represents a direct and fast analytical procedure to determine the assay. Separation is achieved on Waters μ-Bondapak C18, 300 mm × 3.9 mm × 10 μm column in a single wavelength 230 nm. The method has been validated with respect to linearity, limit of quantification, accuracy and robustness. Critical impurity 4-carboxy benzaldehyde (4-CBA) is found to be within the limit of quantification, 8 ppm, accuracy 97–98%, recovery 98 to 101% and resolution 1.97 from the closest peak. The sample solution is stable and can be used safely within 48 h. The method is verified by real sample reaction mixture...

[学科分类] 分析化学