Detection of apoptosis: A review of conventional and novel techniques
[摘要] Apoptosis,orprogrammedcelldeath,hasbeenshowntoplayaroleinanumberofdiseases,includingheartdiseaseandcancer.Apoptosisisalsoavitalcellularprocessthathelpstoregulatetissuegrowth,fetaldevelopment,immuneresponse,andahostofotherbiologicalprocesses.Deviationfromthecarefulregulationofapoptosisisresponsibleforahostofdiseasesandhealthconcerns.Assuch,understandingtheprocessofapoptosisisvitalfortherapydevelopment.Overtheyears,anumberofmethodshavebeendiscoveredanddevelopedtodetectapoptosis.Thereareseveralstandardtechniquessuchaselectronmicroscopy,theTUNELassay,andflowcytometry.Inaddition,newtechniquesarequicklyemerging,suchasmicrofluidicdevices,singlemoleculespectroscopy,andelectrochemicalmethods.Thisreviewwillcoversomeexamplesofthemostcommontechniquesaswellassomenewtechniquesinordertoshowthebroadspectrumofmethodsavailabletodetectapoptosisincells...
[发布日期] [发布机构]
[效力级别] [学科分类] 分析化学
[关键词] [时效性]
