The lmo0501 gene coding for a putative transcription activator protein in Listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions

[摘要] In L. monocytogenes EGDe, the lmo0501 gene locus encodes a protein similar to the mannitoltranscription regulator (MltR) protein in B. subtilis and B. stearothermophilus. In this studywe investigated its functional role in L. monocytogenes EGDe cells in view of growth underdifferent stress conditions. Increased lmo0501 gene expression at mRNA level was detected inresponse to cold, osmotic and organic acid stress exposure. An EGDe Δlmo0501 mutant strainwas diminished in growth compared to the wild type strain in minimal defined mediumcontaining either glucose or fructose, as carbon sources. Furthermore the lmo0501 null mutanthad retarded growth under cold (4°C), salt (NaCl) and organic acid stress conditionscompared to the parental wild type strain. Our results confirm the role of the lmo0501 gene inview of adaptation of L. monocytogenes cells to stress conditions as well as a contribution tothe efficient utilization of glucose and fructose as carbon sources.

[发布机构] University of Zurich

[效力级别] 570 Life sciences

[关键词] Institute of Food Safety and Hygiene;570 Life sciences;biology;610 Medicine & health