Effect of Ensure® overfeeding on the anorectic effect of amylin

[摘要] The aim of this study was to investigate if amylin;;s anorectic effect depends on diet composition and on the length of exposure to high caloric diets. Our investigations resulted in the following findings and conclusions. First study Findings: After three weeks voluntary Ensure® overfeeding, obese rats showed a significant anorectic response after central acute (5pmol) or chronic (2pmol/h) amylin. In addition, amylin-infused rats lost significantly more body weight than the control saline rats. Conclusion: Long-term access to Ensure® does not cause insensitivity to central amylin in obese rats. Second study Findings: After three days of voluntary Ensure® feeding, amylin injection (acute, central: 5pmol or peripheral: 5µg/kg), did not lower energy intake in rats that were still provided with access to Ensure®. However, amylin insensitivity was reversed by increasing the central dose to 10 or 100pmol, or by switching rats back to chow for three days. At that point, amylin-injected rats showed a significant decrease in energy intake similar to controls.Conclusion: Short-term Ensure® exposure causes amylin insensitivity, which is dose-dependent and reversible. Third study Findings: After three days of chow, high fat or Ensure® neutral access, amylin-injected rats (acute, central: 5pmol) ingested significantly less. Rats with access to Ensure® cacao for three days did not lower their intake after amylin.Conclusion: Amylin insensitivity after exposure to Ensure® seems to be more dependent on the palatable flavor, than diet composition.

[发布机构] University of Zurich

[效力级别] 570 Life sciences

[关键词] Institute of Veterinary Physiology;570 Life sciences;biology