INVESTIGATIONS OF THE BIOSYNTHESIS OF ELAIOMYCIN

[摘要] The biosynthesis of the antibiotic elaiomycin (1), produced by Streptomyces gelaticus, has been investigated. Precursor incorporation studies using ((;;13)C)- and {(;;3)H,(;;14)C}-labeled forms of serine have shown that carbons 2-4 of elaiomycin are derived from serine, with the hydroxymethyl carbon of serine being incorporated into C-4 of elaiomycin. An administration of (methyl-(;;13)C)-methionine has proven the methyl carbon of methionine to be the source of the O-methyl carbon of elaiomycin. ((;;13)C)- and {(;;14)C}-labeled precursor incorporation experiments indicate the novel introduction of C-2 of acetate into C-1 of elaiomycin. The administration of precursors labeled with contiguous (;;13)C and (;;15)N atoms has shown the oxygen-bearing nitrogen atom in elaiomycin to be derived from n-octylamine and the other nitrogen atom to be derived from serine.

[发布机构] Rice University