Effect of a novel antiplatelet agent, IC, on mural thrombogenesis in human blood perfused over a collagen coated surface

[摘要] Epifluorescent video-microscopy was used to evaluate the effects of a novel antiplatelet agent, IC, on platelet adhesion and aggregation in the formation of mural thrombi. Studies demonstrate that compound concentration and shear rate influence the drug effectiveness. Whole blood, treated with that compound, at concentrations varying from 25 $mu$mol/l to 200 $mu$mol/l, and at 100/s and 1000/s shear rates, was perfused over collagen coated coverslips in a parallel plate flow chamber. At 1000/s wall shear rate, the compound inhibits total platelet accumulation from 34% to 81%, depending on the drug concentration used. Whereas at 100/s shear rate and 100 $mu$mol/l, the inhibition varies from 34% to 57% depending on the flow time. Platelet aggregation and adhesion are affected by the drug at all concentrations and shear rates; however results show that adhesion is less affected than aggregation and that platelet accumulation is kinetically much slower at the lower wall shear rates.

[发布机构] Rice University