Analysis of regulatory mechanisms of genes controlled by the transcription factor NF-kappaB

[摘要] NF-kappaB transcription factors are central to the regulation of many vital processes including immune response. It is known from microarray measurements and clustering methods that NF-kappaB dependent genes in humans are expressed in functionally distinct ;;waves;;. This research helps determine how the expression of these sequences is a consequence of their structure, molecular constitution and evolution. This thesis identifies location of TF-binding sites and consensus regions in the DNA sequences that are upregulated by NF-kappaB and examines their structure. This project uses a variety of tools and databases available for sequence analysis including Blast, BLAT, MATCH, GeneBee, BioProspector and MEME. This analysis is one aspect of the larger ;;Investigation of NF-kappaB Pathways;; project underway at UTMB, Galveston and Rice University. Future promoter analysis of these results will verify the location of functional regulatory sites, thereby enabling us to postulate and verify a model governing expression of NF-kappaB dependent genes.

[发布机构] Rice University

[效力级别] Statistics