ASSAY FOR THE MAJOR DOG ALLERGEN, CAN F I - INVESTIGATION OF HOUSE DUST SAMPLES AND COMMERCIAL DOG EXTRACTS

[摘要] Monospecific rabbit antibodies were used to develop a sensitive two-site enzyme immunoassay to measure a major dog hair and dander allergen, Can f I. This Can f I assay demonstrated no reaction with 17 heterologous allergen sources, including dog albumin, cat, guinea pig, and horse. Analysis of serial dilutions of purified Can f I and the international standard for dog was parallel. The assay was considered specific for Can f I with a lower limit of detection at 0.03-mu-g/ml. Total imprecision was from 2% to 6%. Commercial dog extracts for specific immunotherapy contained from 0.7 to 290-mu-g of Can f I per milliliter. The assay was used to measure Can f I in 136 house dust samples collected from 103 homes across the United States. Concentration of the dog allergen was expressed as micrograms of Can f I per gram of dust. Prevalence of Can f I in the dust samples ranged from < 0.3 to 10,000-mu-g/gm. Serial dilutions of samples containing Can F I were paralle to the standard. The median Can f I value for homes with a dog in residence was 120-mu-g/gm, and for homes with no dog, 3-mu-g/gm. With few exceptions, homes with no dog in residence had < 10-mu-g/gm. This Can f I assay will provide useful information for assessing commercial extracts as well as monitoring dog-allergen exposure and allergen-control methods.

[发布日期] 1991-12-01

[关键词] ENVIRONMENTAL ALLERGEN;AEROALLERGEN;DOG HAIR AND DANDER;ELISA;RABBIT ANTIBODY