The synergistic effects of TaAGP . L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development

[摘要] Starch is synthesized from a series of reactions catalyzed by enzymes. ADP-glucose pyrophosphorylase (AGPase) initiates the synthesis pathway and synthesizes ADP-glucose, the substrate of starch synthase (SS), of which SSIV is an isoform. Mutations of the AGPase subunit and SSIV-coding genes affect starch content and cause variation in the number of granules. Here, we pyramided the functional mutation alleles of the AGPase subunit gene TaAGP . L-B1 and the SSIV-coding gene TaSSIVb-D to elucidate their synergistic effects on other key starch biosynthesis genes and their impact on starch content. Both the TaAGP . L-B1 and TaSSIVb-D genes were expressed in wheat grain development, and the expression level of TaAGP . L-B1 was higher than that of TaSSIVb-D . The TaAGP . L-B1 gene was downregulated in the agp . L-B1 single and agp . L-B1/ssIV-D double mutants at 12 to 18 days after flowering (DAF). TaSSIVb-D expression was significantly reduced at 6 DAF in both ssIV-D single and double mutants. In the agp . L-B1/ssIV-D double mutant, TaGBSSII was upregulated, while TaAGPSS , TaSSI , and TaSBEII were downregulated. Under the interaction of these genes, the total starch and amylopectin contents were significantly decreased in agp . L-B1 and agp . L-B1/ssIV-D mutants. The results suggested that the mutations of TaAGP . L-B1 and TaSSIVb-D genes resulted in variation in the expression patterns of the other four starch synthetic genes and led to a reduction in starch and amylopectin contents. These mutants could be used further as germplasm for resistant starch analysis.

[学科分类] 急救医学