Genome-wide analysis of microRNAs induced and regulated by colchicine in mulberry

[摘要] A variety of plants were colchicine treated to double their chromosome number. Chromosomes are geneticcarriers that determine the individual traits of organisms. The doubling of chromosomes will lead to modifications inplant morphology, physiology and genetics. To determine the response of mulberry trees induced by colchicine, usingmulberry variety Yu-711 leaves as research materials, two small RNA libraries (control and experimental groups) wereconstructed. It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results.A comparison of data between the control group and the experimental group revealed 37 differentially expressedmiRNA genes, 19 genes of which were up-regulated and 18 genes of which were down-regulated. Eight miRNAs wereselected from 37 differentially expressed miRNA genes. These miRNAs were verified by quantitative real-time PCR,and the expression levels of these miRNAs were found to be consistent with those obtained by sequencing, whichproved the accuracy of sequencing results. The PsRNATarget software was used to predict the target genes of 8miRNAs. The Gene Ontology and Kyoto Encycopedia of Genes and Genomes analysis were used to collect thefunctions of target genes and confirm that target genes are mainly involved in biological processes, cell componentsand molecular functions. novel-77 miRNA was selected from 37 differentially expressed miRNAs. What will serve as afundamental data in understanding mulberry miRNAs function in molecular biology research and further providesthe molecular mechanism of mulberry gene regulation as induced by colchicine is that the prediction of novel_77precursor sequence and the target gene (XM_010104258.2), as well as the secondary structure analysis.

[学科分类] 仪器

[关键词] Chromosomes;Novel_77;qRT-PCR;Target genes;Colchicine