Transient and DNA-free in vivo CRISPR/Cas9 genome editing for flexible modeling of endometrial carcinogenesis

[摘要] The Clustered Regularly Interspaced Short PalindromicRepeats (CRISPR) and CRISPR-associated protein 9 (Cas9)(CRISPR/Cas9)-mediated generation of somatically genetically engineered mouse models have emerged as a newapproach for in vivo modeling of cancer [1]. Here, wedescribe a novel DNA-free, easy, rapid, flexible, multiplexable, and robust method to model endometrial neoplasiaby CRISPR/Cas9 ribonucleoprotein (RNP) electroporationinto the uterus of mice.

[学科分类] 社会科学、人文和艺术（综合）