Comparative Analysis Of Effect Of Various Mobile Phase Solvent Systems In Forensic Examination Of Pepper Spray Residues By HPTLC.

[摘要] In the present study the effect of lead stress on the primary (shikimic acid) and secondary(phenylpropanoids) metabolic pathways was investigated in leaves of garden cress (Lepidium sativum L.). Theproduction of hydrogen peroxide (H2O2) and malondialdehyde (MDA) increased under lead stress in leaves ofgarden cress. The activity of NADH-oxidase (EC: 1.6. 3.3) is elevated continuously and correlated with theincrease of both H2O2 and MDA. Shikimic acid, the total phenolics, the total flavonoids and anthocyaninaccumulated in response to lead stress.The activity of NADP-shikimate dehydrogenase (SKDH, EC 1.1.1.25)increased and correlated with the enhancement of shikimic acid production. The activities of phenylalanineammonia lyase (PAL, EC: 4.3. 1.24), chalcone synthase (CHS, EC: 2.3.1.74) and chalcone isomerase (CHI, EC:5.5.1.6) raised under the same treatment and correlated with the increase of total phenolics, total flavonoidsand anthocyanin.The antioxidant activity in leaves was induced by lead stress in a concentration-dependentmanner as judged by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidantpower (FRAP) assay. Thus, it seems likely that the application of abiotic stresses is a valid strategy to enhancethe primary and secondary metabolism of plants. If certain specific primary or secondary metabolites wererequired, it would be suggested to apply stresses to hasten their biosynthesis.

[学科分类] 工程和技术（综合）

[关键词] Lead;Lipidium sativum;Hydrogen peroxide;Malondialdehyde;Shikimic acid;Phenolics;Flavonoids;Anthocyanin;Enzymes.