The Synergistic Effect of a Combination of Anise Seed Extract and Antibiotics (Erythromycin, Ampicillin, Gentamycin, Amikacin) against the Escherichia Coli Bacterium.

[摘要] The combination of plant extracts with antibiotics as resistance to microorganisms that have shownresistance to many synthetic drugs has been ongoing for a number of years. In this study, the interactionbetween ethanolic extract of anise seeds and four antibiotics were investigated and the inhibition effects offour antibiotics and the crude extract of anise seeds against a strain of E. coli were studied individually. Resultsshow that the zone of inhibition was20mm for Gentamycin, 19mm for Amikacin, and 11mm for tetracycline,while the E. coli showed resistance to the antibiotics of Ampicillin. Furthermore, the zone of inhibition whenthe extract alone was used was 10 mm at a concentration of 250 mg/ml and 20 mm at 500 mg/mlconcentration. The bacterium showed resistance at a concentration of 1000 mg/ml. However, their synergisticeffect was more efficient and induced an inhibition zone of 21.5mm in diameter. The significant zone ofinhibition was reported as 21.5mm when the E. coli was exposed to a combination of Amikacin with 1000mg/ml concentration of anise seed extract followed by 21.2mm at a concentration of 500 mg/ml with the sameantibiotic and 21.1mm at a concentration of 250 mg/ml. A combination of Gentamycin with anise seed extractat the same concentrations revealed zones of inhibition as 20.2 mm, 20.1 mm and 20.1 mm respectively. Acombination of the anise seed extract with tetracycline did not result in the same inhibition zone, beingapproximately 11.2 mm at all three concentrations. However, high resistance was shown when the E. colibacterium was exposed to Ampicillin at the three different concentrations of anise seed extract. The results ofthe present study illustrate that this extract can be used alone or combined with antibiotics to fight multi-drugresistant bacteria such as the E. coli bacterium.

[学科分类] 工程和技术（综合）

[关键词] Synergistic effects;anise seed;Erythromycin;Ampicillin;Gentamycin;Amikacin