Cytotoxicity of chlorambucil immobilized on magnetic iron oxide nanoparticles Fe 3 O 4

[摘要] Chlorambucil is a nitrogen mustard alkylating agent used for the treatment of various malignant and non-malignant cancers. The use of the chemotherapeutic is limited by its toxic side effects which precludes the use of optimal doses. It was decided to investigate if the immobilization of chlorambucil on the magnetic nanoparticles Fe 3 O 4 would increase its cytotoxicity toward chosen cancerous cell lines: ‘Jurkat’ and ‘Hut 78’. The drug was attached to the nanoparticles coated with (3-aminopropyl)triethoxysilane. The obtained nanostructures were characterized using IR spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and dynamic light scattering (DLS) technic. Zeta-potential measurements at various pH were also carried out. The drug release studies showed that chlorambucil is released from the nanoparticles surface very effectively. The cytotoxicity studies demonstrated that chlorambucil immobilized on the magnetic nanoparticles Fe 3 O 4 displays significantly higher cytotoxicity in comparison to its unbound form.

[学科分类] 计算机科学（综合）