Ex VitroSeedling Development fromIn VitroRhizome-Like Bodies inEulophia promensisLindl.: A New Technique for Orchid Propagation

[摘要] This communication describesin vitroseed germination, embryo differentiation, andex vitroseedling production fromin vitrorhizome-like bodies of a terrestrial orchid,Eulophia promensisLindl. Effects of two nutrient media, namely, Murashige and Skoog (MS) and Phytotechnology Orchid Seed Sowing medium (P723) supplemented with 6-benzylaminopurine (BAP; 0.5–1.0 mgL−1) and/orα-naphthalene acetic acid (NAA; 0.5–1.0 mgL−1) and activated charcoal (2.0 gL−1), were studied on seed germination and subsequent development of embryos. Maximum seed germination (100%) was recorded in P723medium fortified with 1.0 mgL−1BAP + 2.0 gL−1activated charcoal. The different developmental stages of protocorm morphogenesis were traced out. In subsequent subcultures, the protocorms proliferated profusely and developed rhizome-like bodies (RLBs) with numerous hair-like structures. These RLBs were transferred to pots containing potting mixture composed of humus + coir dust + saw dust (1 : 1 : 1) where ∼80% of RLBs survived and produced 1–3 seedlings per RLB. This is the first time report forin vitrogermination of seeds andex vitroseedling production fromin vitroraised RLBs inEulophia promensis. This is a time saving and cost effective protocol that could be extended to other economically important, rare, and endangered orchids for propagation and conservation.

[学科分类] 植物学