In VitroAntioxidative Evaluation ofα- andβ-Carotene, Isolated from Crude Palm Oil

[摘要] The present work describes the isolation ofα- andβ-carotene from crude palm oil and their antioxidant potential in anin vitromodel. Pure product was isolated by the method adopted. Antioxidant activities of the isolatedα- andβ-carotene were analyzed in five different concentrations of 0.001, 0.005, 0.01, 0.05, and 0.1% (w/v). From the several assays conducted, an observation was made that the antioxidant activity of the product shifted between antioxidant and prooxidant effects depending on the concentration and the system analyzed. The metal chelation, DPPH radical scavenging, and superoxide scavenging activities showed almost similar results in terms of high activity at lowest concentrations. ABTS-scavenging activity was displayed only by a particular antioxidant concentration of 0.1%. Lipid peroxidation assay pronounced the activity of 0.1% antioxidant in inhibiting oxidation of sensitive bioactive lipids.In vitroantidenaturation test again specified the efficacy of low concentrations in preventing protein denaturation. Through this study a definite dosage formulation for consumption of carotenoids is being proposed which will enhance health promotion and prevent chronic diseases when taken as fortified foods or dietary supplements.

[学科分类] 分析化学