Enhanced Production and Stability of Laccase Using Some Fungi on Different Lignocellulosic Materials

[摘要] Thepotentialsofthirteenlignocellulosicwasteswheatbran,barleybran,coneofPinusnigra,sawdust,cornbran,oat,ricebran,canola,driedtea,groundcloverstraw,sunflowerstalk,soybeanbagasse,drieddistillersgrainswithsoluble(DDGS)assolidsubstratesinrespectoflaccaseproductionbythewhite-rotfungiTrametesversicolor(ATCC200801),Phanerochaetechrysospsorium(ME446)andPleurotussajor-cajuundersolid-stateconditionswereassessed.ThehighestlaccaseactivitywasdetectedwhenT.versicolorwasgrownongroundcloverstraw,showingamaximumvalueof22.36Uml-1.Thehighenzymeactivitywasobservedtocorrespondwithchemicalcompositionofsubstrateevaluatedinrespectofholocellulose,lignin,carbonandnitrogencontents.Inaddition,FTIRanalysiswasperformedtochemicalchangesofsubstratebeforeandafterusinginsolidstateculture.Furthermore,pHandthermalstabilityofenzymeobtainedfromT.versicolorATCC200801grownoncloverstrawwerestudied.Also,effectsofdifferentinhibitorsonthesameenzymeactivitywereinvestigated.

[学科分类] 生物科学（综合）

[关键词] Solid state fermentation;laccase;FTIR;white rot fungi;stability