Studies on the Second Component of Complement

[摘要] 1. 1. The conversion of EAC'1,4 to EAC'1,4,2 by purified C'2 has been investigated with respect to its kinetics and the principal factors influencing this reaction. A mathematical theory has been developed and its applicability evaluated.2. 2. From kinetic evidence and on the basis of titration data, the transformation of EAC'1,4 to EAC'1,4,2 has been shown to involve a single reaction step, which means that interaction of one SA2C'1,4 site with C'2 converts a cell to the state EAC'1,4,2. It follows that hemolysis by antibody and complement is a one-hit or noncumulative process.3. 3. On the basis of these results a method has been developed for measurement of C'2 in molecular terms.4. 4. The limiting-component principle of Hegedus and Greiner has been shown to be invalid. The over-all “titer” of complement depends primarily on the supply of C'2, but the availability of C'1, C'4 and the C'3 factors also plays a role because their concentrations influence the efficiency with which C'2 is utilized.

[学科分类] 生物科学（综合）