Evaluation of an Automatic Calcium Titrator

[摘要] An automatic calcium titrator for determining total serum calcium concentration has been evaluated. The instrument incorporates a motorized buret, a fluorometer, and a digital readout that is responsive to the quenching of the fluorescent calcium—calcein complex by the chelating agent, ethyleneglycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid. Analyses made with the calcium titrator were compared with those made with the SMA 12/60 and atomic absorption spectrophotometry. Good correlation was obtained in each instance. Slight hemolysis and bilirubin concentrations near normal did not affect the results; however, increased concentrations of these substances resulted in decreased values. The precision of analysis depends on the technique used in pipetting the sample. A single analysis of 0.1 ml of serum can be completed in 1 to 2 min. With careful analytical technique, precision is good (CV, 0.72%).

[学科分类] 过敏症与临床免疫学