A Simplified Radioimmunoassay for Human Pituitary Luteinizing Hormone in Serum

[摘要] A simplified radioimmunoassay is described for human pituitary luteinizing hormone (LH). The principal modification over existing methods is that talc is used to separate antibody-bound hormone from free hormone by adsorption of the latter. Serum concentrations of LH in normal males, ovulatory females (follicular phase), postmenopausal females, patients with primary gonadal failure, and patients with hypopituitarism averaged 4.7, 4.6, 26.4, 30.8, and 1.9 µg of reference std. LER 907 per 100 ml of serum, respectively (10.3, 10.1, 57.8, 67.5, 4.2 milli-int. units/ml, respectively). These values are consistent with the clinical diagnoses.

[学科分类] 过敏症与临床免疫学