Determination of Plasma α-Amino Nitrogen by Atomic Absorption Spectrophotometry

[摘要] An uncomplicated, indirect method is described for the determination of α-amino nitrogen in plasma. Plasma is deproteinized with trichloroacetic acid and the protein-free filtrate is neutralized with alkali. The α-amino groups complex with and solubilize copper from insoluble copper phosphate. The solubilized copper, measured by atomic absorption spectrophotometry, is proportional to the amount of α-amino nitrogen present in the plasma.

[学科分类] 过敏症与临床免疫学