Cloning and sequencing of the 5′ region of the human glucose‐6‐phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells

[摘要]

We have cloned and sequenced the first 1.2 kb of the 5′ region of the human glucose-6-phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity. Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.

[学科分类] 生物化学/生物物理

[关键词] Glucose-6-phosphatase;Dexamethasone;Insulin;Gene regulation;cAMP;C/EBP;CCAAT/enhancer binding protein;HNF;hepatocyte nuclear factor;CRE;cAMP response element;IRE;insulin response element;GRE;glucocorticoid response element