Production and crystallization of MHC class I B allele single peptide complexes

[摘要]

Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele complexes. All crystallize in the space group P2₁2₁2₁, with similar unit cell dimensions of approximately , contain one complex per asymmetric unit and diffract to approximately 2.0 Å resolution.

[学科分类] 生物化学/生物物理

[关键词] Protein expression;MHC class I;HLA B allele;X-ray crystallography;Peptide complex