Evidence for different binding sites on the 33‐kDa protein for DCMU, atrazine and QB

[摘要]

Two DCMU-resistant strains of the cyanobacterium Synechocystis 6714 were used to analyse the binding sites of DCMU, atrazine and Q_B. DCMU^r-II_A was DCMU and atrazine resistant; it presented an impaired electron flow and its 33-kDa protein was weakly attached to the membrane. DCMU^r-II_B, derived from the former, simultaneously regained atrazine sensitivity, normal electron flow and a tight linkage of the 33-kDa protein to the membrane. This mutant shows that loss of DCMU binding does not necessarily affect the binding of either atrazine or Q_B. The role of the 33-kDa protein is discussed.

[学科分类] 生物化学/生物物理

[关键词] Cyanobacteria;Mutant;Herbicide;Photosynthesis;Photosystem II;DCMU;3-(3;4 dichlorophenyl)-1;1-dimethylurea;DCPIP;2;6-dichlorophenolindophenol;Wt;wild type;PS II;Photosystem II;BSA;bovine serum albumin