Calvasculin, as a factor affecting the microfilament assemblies in rat fibroblasts transfected by src gene

[摘要]

Cell transformations accompany alterations in cell morphology and microfilament patterns. Calvasculin encodes mRNA termed pEL-98, 18A2, 42A, p9Ka, or mtsl, found to be elevated in several metastatic cell lines. We report the elevation of calvasculin expression in SR-3Y1 cells, which show disappearance of ordered microfilaments, compared to that in 3Y1 cells and that the similar distribution of calvasculin to that of actin filaments. Interestingly, calvasculin co-sediments with F-actin and bundles actin filaments in a Ca²⁺-dependent manner. This activity, along with the elevation of calvasculin following transformation, suggests that the disorganization of filaments in SR-3Y1 cell is due to the cross-linking activity of calvasculin.

[学科分类] 生物化学/生物物理

[关键词] Calvasculin;EF-hand;F-actin;Microfilament;Rat fibroblasts transfected by src gene;SR-3Y1 cell;EGTA;ethylenebis(oxyethylenenitrilo) tetraacetic acid;Tris;2-amino-2-(hydroxymethyl)-1;3-propanediol;PBS;phosphate-buffered saline