Molecular cloning of a human gene (S31) encoding a novel serotonin receptor mediating inhibition of adenylyl cyclase

[摘要]

We report the molecular cloning of human gene (S31) containing an open reading frame of 1095 nucleotides, which encodes a protein of 365 amino acids. The encoded protein contains seven hydrophobic putative transmembrane domains considered the hallmark of G protein-coupled receptors. The amino acid sequence shows highest homology to receptors for serotonin (5-hydroxytryptamine). Expression of this receptor in murine Ltk ⁻ cells conferred upon these cells the ability to respond to serotonin by inhibition of adenylyl cyclase. No response was observed to isoproterenol, epinephrine, histamine, dopamine or melatonin in the transfected cells. We propose that the human gene S31 encodes a novel serotonin receptor.

[学科分类] 生物化学/生物物理

[关键词] Serotonin;5-Hydroxytryptamine;Receptor (human gene);Adenylyl cyclase;Inhibition;5-HT;5-hydroxytryptamine;serotonin;BSA;bovine serum albumin;HEPES;4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid;kb;kilobase(s);MEMα;minimal essential medium;alpha-modified;nt;nucleotide(s);ORF;open reading frame;SDS;sodium dodecylsulphate;Solutions;BSS;126 mM NaCl;0.64 mM KCl;98 μM MgCl2;5 μM CaCl2 and 14.5 mM Tris-HCl;pH 7.5;1 × Denhardt's solution;0.02% Ficoll;0.02% polyvinylpyrrolidone and 0.02% bovine serum albumin;1 × SSC;150 mM NaCl and 15 mM sodium citrate