Effects of anti‐triadin antibody on Ca2+ release from sarcoplasmic reticulum

[摘要]

The monoclonal antibody, mAb GE 4.90, raised against triadin, a 95 kDa protein of sarcoplasmic reticulum (SR), inhibits the slow phase of Ca²⁺ release from SR following depolarization of the T-tubule moiety of the triad. The antibody has virtually no effect on the fast phase of depolarization-induced Ca²⁺ release nor on caffeine-induced Ca²⁺ release. Since the slow phase of depolarization-induced Ca²⁺ release is also inhibited by dihydropyridines (DHP), these results suggest that triadin may be involved in the functional coupling between the DHP receptor and the SR Ca²⁺ channel.

[学科分类] 生物化学/生物物理

[关键词] Triadin;Excitation-contraction coupling;Ca2+ release;Sarcoplasmic reticulum;DHP;dihydropyridine;DIR;depolarization-induced Ca2+ release;e-c coupling;excitation-contraction coupling;HEPES;N-2 hydroxyethyl piperzine-N-2 ethanesulfuric acid;JFP;junctional foot protein;mAb;monoclonal antibody;MES;2-(N-morpholino) ethanesulfonic acid;PMSF;phenylmethyl sulfonyl fluoride;SR;sarcoplasmic reticulum;TC;terminal cisternae;T-tubule;transverse tubule