Phorbol ester‐induced secretion of human hepatocyte growth factor by human skin fibroblasts and its inhibition by dexamethasone

[摘要]

Human skin fibroblasts secreted a certain amount of human hepatocyte growth factor (hHGF), as determined by an enzyme-linked immunosorbent assay for hHGF. This hHGF secretion was remarkably stimulated by protein kinase C (PKC)-activating phorbol esters, which was inhibited by the simultaneous addition of dexamethasone. Pretreatment with phorbol 12-myristate 13-acetate (PMA) caused a down-regulation in hHGF secretion. hHGF secreted by the PMA-treated cells showed a potent hepatocyle growth-promoting activity which was neutralized by an anti-hHGF antiserum. These results indicate both that PMA-treated human skin fibroblasts produce biologically active hHGF and the possible involvement of PKC activation in this process.

[学科分类] 生物化学/生物物理

[关键词] Human hepatocyte growth factor;Phorbol ester;Protein kinase C;Human skin fibroblast;Dexamethasone;DNA synthesis;hHGF;human hepatocyte growth factor;HGF;hepatocyte growth factor;PMA;phorbol 12-myristate 13-acetate;PDBu;phorbol 12.13-dibutyrate;4α-PDD;4α-phorbol 12;13-didecanoate;PKC;protein kinase C;hSF;human scatter factor;MEM;(Eagle's) minimum essential medium;ELISA;enzyme-linked immunosorbent assay;BSA;bovine serum albumin;PBS;phosphate-buffered saline