Transfer of tightly‐bound tritium from the chloroplast membranes to CF1 is activated by the photophosphorylation process

[摘要]

Isolated thylakoids were incubated for 14–16 h in the buffer containing 3-6 mCi T₂O/ml and then resedimented and suspended in non-radioactive medium. It was found that illumination of thylakoids induced an increase in radioactivity level in CF₁ isolated from these thylakoids. Such effect was observed only if photophosphorylation substrates (ADP and phosphate or arsenate) were added to the medium during illumination. The light-induced ADP and arsenate-dependent incorporation of tritium into CF₁ was suppressed by DCCD and inhibited by low gramicidin concentrations to the same extent as photophosphorylation.

[学科分类] 生物化学/生物物理

[关键词] Thylakoid;Tritium;Phosphorylation;Membrane;CF1;CF1;catalytic part of the chloroplast ATP-synthase;DCCD;N;N'-dicyclohexylcarbodiimide;PMS;phenazine methosulphate;MV;methylviologen