Membrane lateral pressure as a modulator of glycerol‐3‐phosphate dehydrogenase activity

[摘要]

Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the enzyme activity, namely V _max value. Contrarily, changes in surface charge caused by minute amounts of phosphatidylserine or charged organic substances influenced only the apparent K _m value. The role of bulk phospholipids in regulation of glycerol-3-phosphate dehydrogenase is discussed.

[学科分类] 生物化学/生物物理

[关键词] Membrane lateral pressure;Förster energy transfer;Surface charge;Model membrane;Glycerol-3-phosphate dehydrogenase;Brown adipose tissue mitochondrion;GPDH;glycerol-3-phosphate dehydrogenase;MLP;membrane lateral pressure;Cl2Ind;2;6-dichloroindophenol;TMA-DPH;1-(4-trimethylammoniumphenyl)-6-phenyl-1;3;5-hexatriene;PtdCho;phosphatidylcholine;PtdEtn;phosphatidylethanolamine;PtdSer;phosphatidylserine;ODA;octadecylamine (1-aminooctadecane);DCP;dicetylphosphate