Effects of Cryopreservation on the Cell Viability, Proliferative Capacity andNeuronal Differentiation Potential of Canine Bone Marrow Stromal Cells

[摘要] References(21)We investigated the cell viability, proliferative capacity and neuronaldifferentiation potential of canine bone marrow stromal cells (BMSCs) aftercryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSOand 10% FBS (DF group) or without DMSO and FBS (DF-free group); fresh BMSCs were used as acontrol. The cell viability and proliferative capacity of BMSCs were similar in theDF-free and control groups, while those in the DF group were lower. In all groups, BMSCsdifferentiated into neuron-like cells that stained positive against neuron markers, andthe mRNA expression levels of neuron markers increased after neuronal induction. Inconclusion, cryopreservation with DF-free cryoprotectant solution did not diminish thecell viability, proliferative capacity or neuronal differentiation potential of canineBMSCs.

[学科分类] 兽医学

[关键词] bone marrow stromal cell;canine;cryopreservation;culture;neuron