Detection of monoclonal integration of bovine leukemia virus proviral DNA asa malignant marker in two enzootic bovine leukosis cases with difficult clinicaldiagnosis

[摘要] References(21)Cited-By(1)Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomeswas detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL)without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to3 EcoRI and HindIII fragments in these 2 atypical EBLcattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. Theprobe also hybridized to a large number of EcoRI andHindIII fragments in 5 cattle with persistent leukosis. These resultssuggest that the detection of monoclonal integration of BLV provirus into the host genomemay serve as a marker of monoclonal proliferation and malignancy in difficult to diagnoseEBL cattle.

[学科分类] 兽医学

[关键词] atypical enzootic bovine leukosis;diagnostic marker;monoclonal integration