Terrestrial vertebrates biodiversity form Guanajuato state is poorly known. The purpose of this article is to publish some noteworthy records of amphibians and reptiles and to confirm the presence of one doubtful species. Animals were colected between 2003 and 2006 in diferent localities of San Luis de la Paz, Victoria and Xichu municipalities, belonging to Biosphere Reserve of "Sierra Gorda de Guanajuato", and were deposited at Herpetological scientific collection, at National School of Biological Sciencies, I.P.N. New records of amphibians are: Chaunus marinus, Ollotis nebulifer, and reptiles are: Sceloporus variabilis, Plestiodon tetragrammus, Boa constrictor and Masticophis flagellum. Presence of Lithobates berlandieri is a confirmation of the species in Guanajuato.
